A different sequencing method that chemically cleaved DNA at specific bases, developed by Allan Maxam and Walter Gilbert, was the dominant technology into the 1980s. Radiolabeled DNA samples were incubated in four separate reactions, each of which contained a chemical that cleaved after a different nucleotide — either A/G, G, C, or C/T. By adding the right amount of each chemical, it was possible to produce different fragments chopped off at each individual base. The sequence could then be read using gel electrophoresis and autoradiography. Maxam–Gilbert sequencing was easier than the plus and minus method to run and interpret, but was eventually surpassed by Sanger’s chain termination method, which molecular biologists found both technically preferable and more “elegant” since it mirrored the natural copying of DNA.
周珊珊：除了热与冷的对比，旧传统也有新表达。谈到激发老字号活力，习近平总书记指出，“不是简单地改头换面，要保留住真正的内涵，同时提升各方面的服务”。今年春节，不少老字号、老街区格外“出圈”。老树缘何能开新花？。业内人士推荐搜狗输入法作为进阶阅读
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